Subjects: Biology >> Botany >> Applied botany submitted time 2019-08-26 Cooperative journals: 《广西植物》
Abstract： To study the distribution, diversity and nematicidal activity of endophytic bacteria from mangrove plants collected from Beibu Gulf coast at Xuwen. It can provide bacterial resources for the microbial insecticides. The bacteria were isolated from sixteen samples seven mangrove plants which collected from the Xuwen by designed 10 kinds of isolation medias. The 16S rRNA molecular biology method, dilution coating method and Caenorhabditis elegans screening model were used to explore the diversity and nematicidal activity of endophytic bacteria isolated from mangrove plants. The results showed that 33 endophytic bacteria were isolated by 16 organs and tissues which could be classified into 19 families and 23 genera and the dominant genus were Bacillus. The eight potential new species or genera were obtained. Through the nematicidal activity test obtained 2 kinds of activity bacteria. Two endophytic bacterial had the extremely significant nematicidal activities and the lethal concentration of 50% was 28.63 mg·mL-1 and 178.03 mg·mL-1, respectively. The research confirm that mangrove plants collected from the Xuwen are rich in endophytic bacteria. At the same time, parts of the bacteria have strong nematicidal activity, which could be a potential source for the discovery of the new microbial insecticides.
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